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nf e2p18  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology nf e2p18
    Nf E2p18, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf+e2p18/NF-E2+p18+Antibody/pmc03847324-127-4-17
    Average 93 stars, based on 20 article reviews
    nf e2p18 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    other:

    Article Title: Jun dimerization protein 2 is a critical component of the Nrf2/MafK complex regulating the response to ROS homeostasis
    Article Snippet: Antibodies against Nrf2 (C-20x), NF-E2p18 (MafK; C-16x), α -tubulin (B-7), HO-1 (H-105) and NQO1 (C-19) were from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

    Article Title: Control of Oxidative Stress and Generation of Induced Pluripotent Stem Cell-like Cells by Jun Dimerization Protein 2
    Article Snippet: Antibodies against Nrf2 (C-20), NF-E2p18 (MafK; C-16), α-tubulin (B-7), β-actin (AC-15), HO-1 (H-105), and NQO1 (C-19) were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).

    Article Title: Jun dimerization protein 2 is a critical component of the Nrf2/MafK complex regulating the response to ROS homeostasis
    Article Snippet: The antibodies used were to JDP2 (174 or 249 monoclonal antibody), Nrf2 (Santa Cruz Biotechnology Inc.; C-20x), NF-E2p18 (Santa Cruz Biotechnology Inc.; MafK; C-16x) and pre-immuned rabbit IgG.



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    rabbit polyclonal anti-nf-e2p18  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology rabbit polyclonal anti-nf-e2p18
    Targeting of <t>NF-E2p18</t> but not NF-E2p45 to centromeric heterochromatin. Cells were stained with antibodies against proteins indicated on the left followed by FITC-conjugated secondary antibodies (Left) and DAPI (Center). (Right) The merge of both images. (A and C) Staining of undifferentiated MEL interphase cells. (B) Example of metaphase chromosomes from MEL cells. (Magnifications: ×100.)
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    Image Search Results


    Targeting of NF-E2p18 but not NF-E2p45 to centromeric heterochromatin. Cells were stained with antibodies against proteins indicated on the left followed by FITC-conjugated secondary antibodies (Left) and DAPI (Center). (Right) The merge of both images. (A and C) Staining of undifferentiated MEL interphase cells. (B) Example of metaphase chromosomes from MEL cells. (Magnifications: ×100.)

    Journal:

    Article Title: Nuclear relocation of a transactivator subunit precedes target gene activation

    doi: 10.1073/pnas.211444898

    Figure Lengend Snippet: Targeting of NF-E2p18 but not NF-E2p45 to centromeric heterochromatin. Cells were stained with antibodies against proteins indicated on the left followed by FITC-conjugated secondary antibodies (Left) and DAPI (Center). (Right) The merge of both images. (A and C) Staining of undifferentiated MEL interphase cells. (B) Example of metaphase chromosomes from MEL cells. (Magnifications: ×100.)

    Article Snippet: For immunostainings, the following antibodies were used: rabbit polyclonal anti-NF-E2p18 (C-16; Santa Cruz Biotechnology); rabbit polyclonal anti-NF-E2p45 (C-19; Santa Cruz Biotechnology); rat monoclonal anti-GATA-1 (N6; Santa Cruz Biotechnology); mouse monoclonal anti-polymerase II (H14; Babco , Richmond, CA); goat polyclonal anti-HDAC1 (Santa Cruz Biotechnology); rabbit polyclonal anti-MeCP2 (Upstate Biotechnology, Lake Placid, NY); tetramethylrhodamine B isothiocyanate-conjugated mouse monoclonal anti-Flag (M2, Sigma); FITC-conjugated mouse monoclonal anti-Myc epitope (9E10; Santa Cruz Biotechnology).

    Techniques: Staining

    Relocation of NF-E2p18 after MEL cell commitment to differentiation. (A) Differentiated MEL cells after 4 days of DMSO treatment. Cells were stained as in Fig. ​Fig.1.1. (B) Staining with antibodies against NF-E2p18 of uninduced MEL cells or DMSO-treated, alone or in association with inhibitors of erythroid differentiation, for 12 h. DEX, Dexamethasone; IMID, imidazole. (Magnifications: ×100.)

    Journal:

    Article Title: Nuclear relocation of a transactivator subunit precedes target gene activation

    doi: 10.1073/pnas.211444898

    Figure Lengend Snippet: Relocation of NF-E2p18 after MEL cell commitment to differentiation. (A) Differentiated MEL cells after 4 days of DMSO treatment. Cells were stained as in Fig. ​Fig.1.1. (B) Staining with antibodies against NF-E2p18 of uninduced MEL cells or DMSO-treated, alone or in association with inhibitors of erythroid differentiation, for 12 h. DEX, Dexamethasone; IMID, imidazole. (Magnifications: ×100.)

    Article Snippet: For immunostainings, the following antibodies were used: rabbit polyclonal anti-NF-E2p18 (C-16; Santa Cruz Biotechnology); rabbit polyclonal anti-NF-E2p45 (C-19; Santa Cruz Biotechnology); rat monoclonal anti-GATA-1 (N6; Santa Cruz Biotechnology); mouse monoclonal anti-polymerase II (H14; Babco , Richmond, CA); goat polyclonal anti-HDAC1 (Santa Cruz Biotechnology); rabbit polyclonal anti-MeCP2 (Upstate Biotechnology, Lake Placid, NY); tetramethylrhodamine B isothiocyanate-conjugated mouse monoclonal anti-Flag (M2, Sigma); FITC-conjugated mouse monoclonal anti-Myc epitope (9E10; Santa Cruz Biotechnology).

    Techniques: Staining

    Identification of protein domains involved in targeting of NF-E2. (A) Staining of NIH/3T3 cells with antibodies against NF-E2p18. (B) Expression of a Myc-tagged NF-E2p18; full-length protein (Upper) and deletion of the C-terminal dimerization domain (NF-E2p18DLZ) (Lower). (C) Expression of a Flag-tagged NF-E2p45; full-length protein (Upper) and deletion of the N-terminal transactivation domain (Lower). (D) Localization of NF-E2p18 containing 80 aa of the NF-E2p45 transactivation domain at its N terminus. (Magnifications: ×100.)

    Journal:

    Article Title: Nuclear relocation of a transactivator subunit precedes target gene activation

    doi: 10.1073/pnas.211444898

    Figure Lengend Snippet: Identification of protein domains involved in targeting of NF-E2. (A) Staining of NIH/3T3 cells with antibodies against NF-E2p18. (B) Expression of a Myc-tagged NF-E2p18; full-length protein (Upper) and deletion of the C-terminal dimerization domain (NF-E2p18DLZ) (Lower). (C) Expression of a Flag-tagged NF-E2p45; full-length protein (Upper) and deletion of the N-terminal transactivation domain (Lower). (D) Localization of NF-E2p18 containing 80 aa of the NF-E2p45 transactivation domain at its N terminus. (Magnifications: ×100.)

    Article Snippet: For immunostainings, the following antibodies were used: rabbit polyclonal anti-NF-E2p18 (C-16; Santa Cruz Biotechnology); rabbit polyclonal anti-NF-E2p45 (C-19; Santa Cruz Biotechnology); rat monoclonal anti-GATA-1 (N6; Santa Cruz Biotechnology); mouse monoclonal anti-polymerase II (H14; Babco , Richmond, CA); goat polyclonal anti-HDAC1 (Santa Cruz Biotechnology); rabbit polyclonal anti-MeCP2 (Upstate Biotechnology, Lake Placid, NY); tetramethylrhodamine B isothiocyanate-conjugated mouse monoclonal anti-Flag (M2, Sigma); FITC-conjugated mouse monoclonal anti-Myc epitope (9E10; Santa Cruz Biotechnology).

    Techniques: Staining, Expressing

    Centromeric targeting of transactivation domain-deficient NF-E2p45 mutants Myc-tagged NF-E2p18, full length or mutant (NF-E2p18DLZ), was cotransfected with deletion mutants of NF-E2p45 lacking the first 80 (NF-E2p45DN80) or 206 (NF-E2p45DN206) N-terminal amino acids of the transactivation domain. (Magnifications: ×100.)

    Journal:

    Article Title: Nuclear relocation of a transactivator subunit precedes target gene activation

    doi: 10.1073/pnas.211444898

    Figure Lengend Snippet: Centromeric targeting of transactivation domain-deficient NF-E2p45 mutants Myc-tagged NF-E2p18, full length or mutant (NF-E2p18DLZ), was cotransfected with deletion mutants of NF-E2p45 lacking the first 80 (NF-E2p45DN80) or 206 (NF-E2p45DN206) N-terminal amino acids of the transactivation domain. (Magnifications: ×100.)

    Article Snippet: For immunostainings, the following antibodies were used: rabbit polyclonal anti-NF-E2p18 (C-16; Santa Cruz Biotechnology); rabbit polyclonal anti-NF-E2p45 (C-19; Santa Cruz Biotechnology); rat monoclonal anti-GATA-1 (N6; Santa Cruz Biotechnology); mouse monoclonal anti-polymerase II (H14; Babco , Richmond, CA); goat polyclonal anti-HDAC1 (Santa Cruz Biotechnology); rabbit polyclonal anti-MeCP2 (Upstate Biotechnology, Lake Placid, NY); tetramethylrhodamine B isothiocyanate-conjugated mouse monoclonal anti-Flag (M2, Sigma); FITC-conjugated mouse monoclonal anti-Myc epitope (9E10; Santa Cruz Biotechnology).

    Techniques: Mutagenesis